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Accumulation of rolling cells expressing P- or L-selectin on PSGL-1 constructs. L1–2 cells expressing human P-selectin (A and C) or L-selectin (B and D) were perfused at the indicated wall shear stress over CHO cell monolayers expressing Fuc-TVII, C2GnT, and the indicated PSGL-1 construct. After 4 min of perfusion, the number of L1–2 cells rolling on the monolayer was quantified. The data represent the mean ± SD of five experiments.
