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. 2008 Jun 6;283(23):15799–15806. doi: 10.1074/jbc.M801553200

FIGURE 3.

FIGURE 3.

Phospho-TrkB level regulated in the striatum of the rats administrated with D1 receptor agonist and antagonist. Littermate rats were injected with control vehicle (PBS; 0.25% ascorbic acid-saline), SKF38393 (A and B, SKF; 1 mg/kg in 0.25% ascorbic acid-saline) and SCH23390 (B, SCH;1 mg/kg in 0.25% ascorbic acid-saline) on postnatal day 4. A, the effects of D1 receptor stimulation at several time points on striatum and frontal cortex were estimated by determining phospho-TrkB. Duplicate samples are displayed. B, the effects of D1 receptor activation and inhibition after 3 h of administration on striatum were estimated by determining phospho-TrkB. Triplicate samples are displayed. Representative immunoblots (IB) are shown. Quantifications of phospho-TrkB immunoreactivity in striatum by NIH Image are shown. Note that there was no significant reduction of the neuron (NSE) marker in the striatum in this pharmacological paradigm (data not shown. control: 100 ± 12.9%, SKF38393: 100.7 ± 7.1%, SCH23390: 107.4 ± 14.8%, mean ± S.D.). n = 4; *, p < 0.05. The bars indicate S.D. IP, immunoprecipitation.