TABLE 1.
Smooth muscle myosin species used in this study
| RLC exchanged? | RLC | |
|---|---|---|
| Double-head phosphorylated | ||
| pp | No | p-RLC/p-RLC (native RLC) |
| ppex | Yes | p-RLC/p-RLC (recombinant RLC) |
| pFpF | Yes | p-FLAG-RLC/p-FLAG-RLC (recombinant RLC) |
|
pHpH
|
Yes
|
p-His6-RLC/p-His6-RLC (recombinant RLC)
|
| Single-head phosphorylated | ||
| pFdH | Yes | p-FLAG-RLC/His6-RLC (recombinant RLC) |
|
pdH
|
Yes
|
p-RLC/His6-RLC (recombinant RLC)
|
| Unphosphorylated | ||
| dd | No | RLC/RLC (native RLC) |
| dHdH | Yes | His6-RLC/His6-RLC (recombinant RLC) |
| dFdF | Yes | FLAG-RLC/FLAG-RLC (recombinant RLC) |