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Substrate specificity of TbUAP. Recombinant TbUAP-His6 was incubated with UTP and different sugar-1-phosphate substrates, as indicated, and the products were analyzed by HPLC. A sugar nucleotide product (UDP-GlcNAc) was observed using GlcNAc-1-P (A) but not without GlcNAc-1-P (B) or with GalNAc-1-P, Glc 1-phosphate, or Gal 1-phosphate (C-E, respectively).
