Skip to main content
. 2008 Jun 6;283(23):16147–16161. doi: 10.1074/jbc.M709581200

FIGURE 3.

FIGURE 3.

TbUAP expression is essential for the growth of bloodstream form T. brucei in culture. A, growth curves for wild type cells, subcultured every 2 days. B, growth curves for TbUAP conditional null mutant cells, subcultured every 2 days, grown under permissive (plus tetracycline) conditions. C, growth curves for TbUAP conditional null mutant cells grown under nonpermissive (minus tetracycline) conditions. D, ethidium bromide-stained agarose gel of reverse transcription-PCR products from RNA extracted from TbUAP conditional null mutant cells after 0, 2, 6, 12, and 48 h and 10 days without tetracycline, as indicated. The upper panel shows reverse transcription-PCR products using TbUAP primers, the middle panel is a control without reverse transcriptase, and the lower panel is a control using Dol-P-Man synthetase (DPMS) primers to show equal RNA input.