FIGURE 4.

Subcellular localization of TbUAP. Wild type (A-C) and TbUAP conditional null mutant cells grown with tetracycline (D-F) and without tetracycline for 48 h (G-I) were stained with rabbit anti-GAPDH (a glycosomal marker) and Alexa 488 anti-rabbit (green channel; A, D, and G) and affinity-purified mouse anti-TbUAP and Alexa 594 anti-mouse (red channel; B, E, and H). Merged images are shown in C, F, and I. The merged image in I also includes a 4′,6-diamidino-2-phenylindole stain (blue) for DNA. No significant signals were obtained when primary antibodies were omitted (data not shown).