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Tomato lectin blotting of wild type the TbUAP conditional null mutant extracts. A, extracts of wild type and TbUAP conditional null mutant (TbUAP cKO) cells 0, 24, and 48 h after tetracycline removal (as indicated) were subjected to SDS-PAGE, transferred to nitrocellulose, and probed with biotinylated tomato lectin followed by horseradish peroxidase-streptavidin. B, as in A, except that the tomato lectin inhibitor, chitin hydrolysate, was included with the lectin.
