Skip to main content
. 2008 Jun 6;283(23):16147–16161. doi: 10.1074/jbc.M709581200

FIGURE 8.

FIGURE 8.

Endoglycosidase digestions of sVSG221 from wild type and TbUAP conditional null mutant cells. A, sVSG221 purified from wild type and TbUAP conditional null mutant (TbUAP cKO) cells before (0 h) and after 48 h without tetracycline (48 h) were subjected to SDS-PAGE and Coomassie Blue staining. B, aliquots of wild type sVSG221 were digested with Endo H (that removes only oligomannose N-linked glycans) or PNGase F (that removes all N-linked glycans), and the products were subjected to SDS-PAGE and Coomassie Blue staining. Species containing two, one, and no N-linked glycans were resolved, as indicated. C, aliquots of sVSG221 from TbUAP conditional null cells grown for 48 h without tetracycline were digested with Endo H or PNGase F, and the products were subjected to SDS-PAGE and Coomassie Blue staining. Species containing two, one, and no N-linked glycans were resolved, as indicated.