TABLE 2.

Kinetic parameters of MaPT with three substrates and with d-Trp as inhibitor

Substrate	Km	Vmax	kcat	kcat/Km
	μm	μm/min	min−1	μm−1·min−1
l-Trp	48.88 ± 1.86	3.41 ± 0.04	80.42 ± 0.94	1.65 ± 0.04
5-OH-l-Trp	303.08 ± 25.73	1.43 ± 0.04	33.73 ± 0.94	0.11 ± 0.09
l-Abrine	105.99 ± 21.14	2.70 ± 0.30	63.68 ± 7.08	0.60 ± 0.23
l-Trpa	107.79 ± 5.07	3.56 ± 0.07	83.96 ± 1.65	0.78 ± 0.05
l-Trpb	13.97 ± 1.86	5.63 ± 0.45	132.78 ± 10.61	9.50 ± 0.16
5-OH-l-Trpb	41.20 ± 3.48	1.40 ± 0.04	33.02 ± 0.94	0.80 ± 0.09
l-Abrineb	19.00 ± 3.34	1.19 ± 0.06	28.07 ± 1.42	1.48 ± 0.18
l-Trpa,b	12.14 ± 3.36	4.87 ± 0.70	114.86 ± 16.51	9.46 ± 0.31

^aKinetic data were obtained in the presence of 50 μm d-Trp.

^bDMAPP, kinetic parameters.