FIGURE 6.

Phosphorylation at Tyr⁴⁴⁷³ abolishes the LRP1-Snx17 interaction. A, unphosphorylated and phosphorylated GST-LRP1-CT fusion proteins immobilized on Sepharose beads were incubated with lysates of HEK293 cells expressing HA-tagged Snx17. Bound proteins were analyzed by anti-HA immunoblotting. B, wild type (WT) or mutant myc-LRP1β was expressed together with HA-Snx17 in the presence or absence of v-Src. Anti-Myc immunoprecipitations (IP) were probed for the presence of Snx17 by anti-HA immunoblotting. As controls for expression, whole cell lysates were analyzed by anti-HA and anti-Myc immunoblotting. WB, Western blot.