FIGURE 4.

Effect of hSLC41A1 overexpression in Salmonella. Growth curves of Salmonella strains MM1927, MM281 transformed with pUC18-(empty), and MM281 transformed with pUC18-hSLC41A1 grown in N-minimal medium supplemented with 10 mmol liter^-1 (A), 100 μmol liter^-1 (B), or 10 μmol liter^-1 (C) MgCl₂. Growth curves averaged from three independent experiments for each respective [Mg²⁺]_e and corresponding serial dilutions (bottom) plated on the solid N-minimal medium are shown. D, steady-state [Mg²⁺]_i of bacteria from strains MM1927, MM281 transformed with pUC18-(empty), and MM281 transformed with pUC18-hSLC41A1 measured after a 20-min incubation in nominally Mg²⁺-free or 10 mmol liter^-1 Mg²⁺ containing saline. Mean ± S.E. of three to four independent experiments are given. OD, optical density.