FIGURE 7.
A, temperature sensitivity of SLC41A1-related [Mg2+]i changes in HEK293-(SLC41A1) cells. [Mg2+]i changes were measured in +tet (15 h) HEK293-(SLC41A1) cells incubated in media containing 10 mmol liter-1 Mg2+. Medium temperatures were held at 37, 25, or 40 °C. [Mg2+]i increases obtained after 20 min exposure to the respective temperature condition are given. The inset shows representative original [Mg2+]i recordings. Values are mean ± S.E. of five to seven single experiments. **, p < 0.01 versus control (37 °C). B, [Mg]t determined in -tet and +tet (5 h and 15 h) HEK293-(SLC41A1) cells incubated in Mg2+-free HEK293 medium and in HEK293 medium supplemented with 10 mmol liter-1 Mg2+. Sets of [Mg]t values determined after 20 and 180 min incubation at these [Mg2+]e are shown. Values are mean ± S.E. averaged from three independent measurements.