FIGURE 3.

Fluorescence anisotropy to determine the binding affinity of LGP2 for RNA. A, a summary of the RNAs used to assess binding by LGP2 and parameters for their interactions with LGP2. The R² column denotes the fit of the data to the Hill equation. The n column denotes the Hill coefficient for the binding isotherm. Where values are missing, the binding isotherm did not reach saturation under the conditions tested, and hence the parameters could not be determined. B, binding isotherms for LGP2 interaction with a ssRNA of 27 nt and a dsRNA of 27 bp.