FIGURE 4.

Reconstruction of the monomeric structure of LGP2. A, an electron micrograph of monomeric LGP2 is shown. The scale bar in the micrograph equals 17 nm. B, class averages of all the particles selected from the micrograph without assumption of symmetry are shown. C, Fourier shell correlation using the 0.5 σ criterion suggests a resolution of 28 Å. D, an asymmetric triangle shows a complete three-dimensional sampling of the particles used in the reconstruction. E, two views of the three-dimensional reconstruction of the monomeric LGP2 and the dimensions of the particles are shown.