FIGURE 5.

Knockdown of p53 expression in CHRF cells delays apoptosis and cell death. CHRF-p53-A (black diamonds), CHRF-p53-B (gray triangles), and CHRF-Neg-A (open squares) cells were stimulated with PMA and assayed for cleaved caspase-3 (A and B). Representative data are shown from one experiment on day 7 after PMA stimulation (A). The cells were fixed and permeabilized, gating was done on TO-PRO-3⁺ events to exclude debris, and binding of a PE-conjugated antibody against active caspase-3 was assessed via flow cytometry (A). B, summary of the active caspase-3 detection based apoptosis assay. Error bars represent the S.E. (n = 2). Cell viability was assayed by the 7AAD exclusion assay (C), and apoptosis among viable (7AAD^–) cells was measured by co-staining with Annexin V-PE and 7AAD (D). Error bars, S.E. (n = 2–6).