FIGURE 5.
The ant genes are regulated by quorum sensing. A, strain PAO1, carrying an inducible copy of pqsR and either the PantA-lacZ or the PpqsA-lacZ fusion, was grown at 37 °C in MOPS-buffered LB supplemented with 0.4% l-arabinose for induction of PqsR. Each culture was then assayed for β-galactosidase activity. Shown are representative data from a single experiment. B–D. RT-PCR was used to measure expression of antA (B), antR (C), and pqsR (D) mRNA from the indicated strains grown at 37 °C in MOPS-buffered LB. Error bars show the range of two independent experiments. wt, wild type.