TABLE 1.
Selected genes induced in the Δ prrF1,2 mutant
WT, wild type.
|
Gene ID |
Function of gene product |
WT high iron vs. WT low
irona |
prrF mutants vs. WT, low
ironb |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ΔprrF1 | ΔprrF2 | ΔprrF1,2 | |||||||||
| Tricarboxylic acid cycle | |||||||||||
| PA1562 acnA | Aconitase A | 5.3 | 4.6 | 3.7 | 3.5 | NC | 1.9 | 8.6 | 2.5 | 6.5 | 9.8 |
| PA1581 sdhC | Succinate dehydrogenase | 8.6c | 5.7c | 1.9 | 2.1 | NCc | NCc | 2.1c | NCc | 1.7 | 2.0 |
| PA1582 sdhD | 6.1c | 6.1c | 2.0 | 2.5 | NCc | NCc | 3.2c | NCc | 2.0 | 2.5 | |
| PA1583 sdhA | 4.0c | 4.6c | 2.1 | 2.8 | NCc | NCc | 3.7c | NCc | 2.1 | 2.5 | |
| PA1584 sdhB | 2.8c | 3.5c | NC | 2.1 | NCc | NCc | 4.0c | NCc | NC | NC | |
|
PA1787 acnB |
Aconitase B
|
4.9
|
5.7
|
3.2
|
1.6
|
NC
|
NC
|
NC
|
NC
|
2.3
|
2.5
|
| Anthranilate metabolism | |||||||||||
| PA2507 catA | Catechol degradation | 5.7 | 7.0 | 32.0 | 32.0 | NC | NC | 5.7 | 2.8 | 3.0 | 3.7 |
| PA2508 catC | 6.1 | 4.9 | 27.9 | 24.3 | NC | NC | 64.0 | 1.9 | 2.6 | 3.2 | |
| PA2509 catB | 2.3 | 3.2 | 8.0 | 12.1 | NC | NC | 4.6 | NC | 3.5 | 2.5 | |
| PA2511 antR | AraC-type regulator | 168.9c | 6.5c | 8.6 | 22.6 | 2.3c | NCc | 104.0c | 13.0c | 4.0 | 4.3 |
| PA2512 antA | Anthranilate degradation | 52.0c | 238.9c | 104.0 | 238.9 | NCc | NCc | 512.0c | 111.4c | 2.3 | 2.1 |
| PA2513 antB | 68.6c | 207.9c | 194.0 | 222.9 | 1.9c | NCc | 137.2c | 73.5c | 2.6 | 2.3 | |
|
PA2514 antC |
52.0c |
73.5c |
168.9
|
59.7
|
1.7c |
1.7c |
55.7c |
13.9c |
3.7
|
3.0
|
|
| Anthranilate biosynthesis | |||||||||||
| PA2080 kynU | Kynurenine pathway | NC | NC | 2.0 | 4.9 | NC | NC | 1.5 | NC | 2.3 | 1.9 |
| PA2081 kynB | NC | NC | NC | NC | NC | 1.5 | NC | 0.4 | NC | NC | |
|
PA2579 kynA |
2.3
|
NC
|
NC
|
1.7
|
NC
|
NC
|
NC
|
NC
|
1.7
|
NC
|
|
| Iron storage and oxidative stress | |||||||||||
| PA3531 bfrB | Bacterioferritin | 168.9c | 24.3c | 9.2 | 26.0 | 1.6c | NCc | 3.0c | NCc | 0.7 | 0.6 |
| PA4236 katA | Catalase | 7.5c | 7.0c | 3.0 | 4.0 | NCc | 1.6c | 5.7c | 3.2c | 5.3 | 6.1 |
| PA4366 sodB | Iron superoxide dismutase | 5.3c | 9.8c | 5.7 | 1.4 | NCc | NCc | 4.6c | 1.9c | 4.6 | 4.0 |
| PA4880 | Probable bacterioferritin | 13.0c | 7.5c | 5.3 | 2.3 | NCc | 2.5c | 14.9c | 2.1c | 13.9 | 21.1 |
Ratio of expression in wild type PAO1 grown in iron-replete conditions vs. wild type PAO1 grown in iron-depleted conditions from four independent microarray experiments. p < 0.0001 for the expression ratio of each gene in each experiment as determined by GeneSpring® analysis software, or indicated as NC (no change).
Ratio of expression in the indicated Δ prrF mutant grown in iron-depleted conditions vs. wild type PAO1 grown in iron-depleted conditions from four independent microarray experiments. p < 0.0001 for the expression ratio of each gene in each experiment as determined by GeneSpring® analysis software, or indicated as NC (no change).
Microarray data previously published by Wilderman, et al (8) that has been reanalyzed in conjunction with newly acquired microarray data.