Figure 4.
hCDC5 interacts with core components of the splicing machinery and the spliceosome in vitro. (A) Immunoprecipitations (IP) from a nuclear splicing extract were performed with three monoclonal antibodies [irrelevant monoclonal antibody (AK105), anti-Sm (Y12), and anti-snRNA cap (anti-trimethylguanosine; α-m3G)] and preimmune (PI) or immune (I) hCDC5C antiserum. Immunoprecipitates were blotted with hCDC5C antiserum. (B) hCDC5 or Sm proteins were immunoprecipitated from an in vitro splicing reaction containing labeled β-globin pre-mRNA at 45 or 90 min after addition of pre-mRNA, and 1/10 of the total reactions were run as standards. The identities of the RNA species are indicated to the left.