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. 2008 Jun 6;283(23):16027–16036. doi: 10.1074/jbc.M710606200

FIGURE 1.

FIGURE 1.

Expression of chaperones and htt in transgenic mice. A, immunoblot analysis of brain cortical lysates from wild-type (WT), BAG1 transgenic, N171-82Q (Tg), and BAG1/N171-82Q double transgenic (DbTg) mice at 5 months of age. The blots were probed with rabbit EM48 to detect aggregated (agg) htt (inner bracket) in the stacking gel and soluble (sol) htt in the resolving gel. Blots were subsequently probed with antibodies to Hsp70, Hsp90, Hsp60, and BAG1. Transgenic BAG1 with a FLAG tag and endogenous BAG1 are indicated. The samples were also probed for the neuronal protein synaptophysin to reveal the loading amounts of proteins. B, densitometric quantification of the relative levels of htt and chaperones in transgenic mice. The ratios of htt (aggregated and soluble) or chaperones (Hsp70, Hsp60, and Hsp90) to synaptophysin are presented. *, p < 0.05 compared with wild type (WT).