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Co-expression of chaperones and mutant htt in HEK293 cells. A, HEK293 cells were transfected with N208-120Q htt alone or together with Hsp70, Hsp40, or BAG1. Cells were stained with antibodies to htt and chaperones and visualized via fluorescent microscopy. B, transfected HEK293 cells were analyzed by immunoblotting. The samples were probed with antibodies to htt (mEM48, upper blot), BAG1, Hsp70, and Hsp40.
