FIGURE 7.

Increased htt aggregation in HEK293 cells treated with BAG1 siRNA. A, immunofluorescent staining of HEK293 cells that were transfected with N208-120Q (control) or N208-120Q with plasmids encoding BAG1 siRNA. The cells were examined 3 days after transfection. Note that co-transfection of BAG1 siRNA increased the size and number of htt aggregates. B, Western blot analysis of the expression of transfected htt and endogenous BAG1 in HEK293 cells. BAG1 siRNA transfection reduced the level of endogenous BAG1 and increased the aggregation of transfected htt seen in the stacking gel (bracket). The blots were probed with antibodies to htt (mEM48, upper panel), BAG1 (middle panel), and tubulin (lower panel).