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. 2008 Jun 6;283(23):15988–15996. doi: 10.1074/jbc.M800562200

FIGURE 1.

FIGURE 1.

a, the purity of PrP was estimated by electrophoresis on precast 12% SDS-PAGE followed by staining with Coomassie Blue. b, CD spectrum of PrP (0.25 mg/ml) confirmed native α-helical conformation. c, electrospray ionization mass spectrometry spectrum of PrP collected on a Waters ZMD single quadrupole mass spectrometer operated in positive ion mode. The spectrum shows a single series of peaks corresponding to multiply charged ions confirming high purity of PrP. The labels on the peaks (A21, A22, etc.) indicate the mass/protonation (charge) ratio of the ion species. Deconvolution of the electrospray ionization mass spectrometry spectrum results in a single peak with a maximum of 23102.35 Da (inset), which is in good agreement with the predicted molecular mass of 23102.42 Da.