FIGURE 8.

Acidic pH induces greater mitochondrial Ca²⁺ accumulation in BI-1-overexpressing cells. HT1080-Neo and HT1080-BI-1 cells were pre-loaded with 1 μm Rhod-2 for 1 h and then treated with nigericin (5 μm) in 140 mm K⁺, Ca²⁺-free buffer (pH 5.0, 6.0, and 7.0). A, Rhod-2 fluorescence intensity was measured over time. Data shown represent 16 independent samples. B, the peak of Rhod-2 fluorescence was recorded for several cells (n = 16 cells) (^*, p < 0.05) versus Neo control cells at the same pH). Mitochondrial Rhod-2 Ca²⁺ fluoresence is shown (right).