Identification of critical motif(s) of PSD-Zip45 required for
mGluR1α clustering. Plasmid DNAs listed at the left of each column
were microinjected into COS-7 cells. After 3 hr, the cells were fixed
and stained with appropriate antibodies. (c, e, h, r, o,
r, and t
Inset) Clustering
scores. Final concentrations of plasmid DNAs are as follows: mGluR1α
and its deletion mutants (0.1 mg/ml) and PSD-Zip45 and its deletion
mutants (0.2 mg/ml). mGluR1αΔ1 and mGluR1αΔ2 are mutant forms
lacking residues 1150–1158 (LTPPSPFRD) and 1159–1179
(SVASGSSVPSSPVSESVLCTP), respectively.