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(A) Schematic map of the chimeric mitochondrial luciferase. Lines and bars indicate the noncoding and coding regions [gray, cytochrome c oxidase subunit VIII (COX8); black, HA1; white, luciferase], respectively. Details of the construction strategy are given in Material and Methods. (B) Immunofluorescence image of HeLa cells transiently transfected with mtLuc and stained with the anti-HA1 mAb.
