Phosphorylation of R-Ras mediates the effects of EphB2 on cell
adhesion. (A) Effects of R-RasY66 mutants on cell
adhesion. The 293T cells were transfected with various R-Ras vectors,
together with EphB2, and plated on surfaces coated with fibronectin,
collagen I, or BSA as a control. (B) R-Ras38V Y66F
suppresses the effect of EphB2 on cell adhesion. The constitutively
activated R-Ras38V and its Y66 mutants were cotransfected with EphB2 in
293T cells, and cell attachment was quantitated.