Figure 6.
Phosphorylation of R-Ras mediates the effects of EphB2 on cell adhesion. (A) Effects of R-RasY66 mutants on cell adhesion. The 293T cells were transfected with various R-Ras vectors, together with EphB2, and plated on surfaces coated with fibronectin, collagen I, or BSA as a control. (B) R-Ras38V Y66F suppresses the effect of EphB2 on cell adhesion. The constitutively activated R-Ras38V and its Y66 mutants were cotransfected with EphB2 in 293T cells, and cell attachment was quantitated.