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. 1999 Nov 23;96(24):13819–13824. doi: 10.1073/pnas.96.24.13819

Figure 5.

Figure 5

MKK6 is required for c-Abl-induced apoptosis. NIH 3T3 cells were transfected with a CD20 reporter construct, together with the indicated plasmids. CD20-positive cells were subjected to the TUNEL assay. The percentage of TUNEL-positive cells in CD20-positive cells is presented. (A) Cells were transfected with 6 μg of pMT21-Abl (or its derivatives) or c-Jun, plus 6 μg of pCDNA3, MKK6(A), MKK3(A), or p38(AF). The result represents an average of three independent experiments. (B) Cells were transfected with 4 μg of pMT21-Abl, 4 μg of MKK6(A), and various amount of MKK6HA or MKK3HA. The result represents an average of two independent experiments. All transfections included 0.6 μg of pCMV-CD20 and were balanced with the appropriate empty vector plasmids.