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. 2008 May 18;8:55. doi: 10.1186/1471-213X-8-55

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Copyright © 2008 Bachmann et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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EM analysis reveals defective septate junctions in vari mutants. Epidermis (A-D, I) and tracheae (E-H) of stage 16 embryos. (A, E): vari^MD109/CyO-twi-GFP; (B, F): vari^MD109; (C, G): vari^03953b/CyO-twi-GFP; (D, H): vari^03953b; (I): wild-type. Adherens junctions (black arrowheads) and developing septate junctions (white arrows) can be distinguished in wild-type embryos. The inset in E shows the regularly aligned septa of pleated septate junctions between adjacent cells. Septate junctions are absent in homozygous vari^MD109(B, F) and vari^03953b(D, H) mutant embryos, while adherens junctions are well developed (black arrowheads).