Figure 1.

Rat MIS type II receptor promoter sequence. (A) The sequence of a genomic fragment containing approximately 1.6 kb of the MISRII was subcloned from a 15-kb clone, which was isolated from λEMBL3 rat library and sequenced on both strands. Potential SF-1-binding sites are shown boxed, as are GATA and Sp1 sites. The boxed sequences labeled CAGA and ?? indicate DNase I footprinted regions (see Fig. 4) that are potential binding sites for Smads and some unknown factor, respectively. The arrow indicates the transcription initiation site found by S1 analysis; the first exon is shown with predicted amino acids underneath, and the first intron is shown with uncapitalized letters. The bases are negatively numbered on the right-hand side from the transcription initiation site. The 5′ ends of the promoter fragments used in the luciferase experiments are bracketed ([) and indicated with their names in bold. The 3′ end for all the promoter fragments is indicated by #. These sequences have been submitted to GenBank under the accession number AF092445. Inverted repeats with Alu-like sequences were observed by sequence analysis at −1336 to −1486 and −476 to 576 and are shown underlined in A and aligned in B.