Figure 2.

Transcription initiation site of the Rat MIS type II receptor. The transcriptional start site of the rat MISRII mRNA was mapped by S1 nuclease digestion. RNA from the indicated sources (rat testes, liver, liver poly(A)+, and yeast) was hybridized overnight in solution with a single-stranded radiolabeled DNA probe. The resulting hybrids were digested with S1 nuclease and analyzed by denaturing gel electrophoresis. The band observed indicates the length of the probe protected from digestion by the hybridized mRNA. Sequencing was done with the same primer as that used for making the S1 probe and electrophoresed alongside the S1-digested fragment to indicate the exact nucleotides for transcription initiation.