Figure 5.

Expression of MIS type II receptor promoter/luciferase reporter in R2C cells. The 1.6-kb MISRII promoter and smaller fragments were placed in front of the firefly luciferase gene and used to measure their ability to drive luciferase expression when transfected into R2C cells. The MISRII promoter is schematically drawn above the represented promoter fragments to indicate the footprinted regions of the promoter, which are shown with black boxes and, when recognized by sequence analysis, the sites were labeled with their cognate transcription factor. The numbers shown indicate the number of base pairs from the transcription initiation site, which is indicated with an arrow. The fragments used in the transfection experiments are represented as shaded bars, and the relative size of each is shown with their names. Cells were transfected with the indicated plasmids in triplicate and the average-fold increase over the promoterless parental vector of four different experiments is plotted to the right of each with error bars representing SEM. Luciferase activity from each of the promoter/reporter plasmids was measured and normalized for transfection efficiency with the activity of a cotransfected thymidine kinase promoter/Renilla luciferase reporter.