The PKL transcript is expressed throughout the plant.
Ribonuclease protection assays were performed to determine the level of
the PKL transcript in the root, rosette, inflorescence,
and siliques of Arabidopsis. To show that the probe used
was specific for PKL, a ribonuclease protection assay
with the same probe was performed with RNA isolated from a wild-type
plant and a plant carrying a deletion allele of PKL,
pkl-9 (Right). A probe for
the cyclophilin transcript ROC3 was used as a positive
control.