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. 2008 May 16;8:50. doi: 10.1186/1472-6750-8-50

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Copyright © 2008 Huang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SDS-PAGE silver staining for purification of BChE/hSA fusion protein from the milk of the transgenic goat, 2177. 1 μg of samples were loaded onto each lane of a precast 4–20% Tris-Glycine polyacrylamide gel under denaturing and reducing conditions in the following order: lane 1, molecular weight marker (Bio-Rad, Mississauga, ON, Canada); lane 2, HQ 50 column load; lane 3, HQ50 column flow-through; lane 4, HQ50 column elution; lane 5, HQ50 column cleaning; lane 6, procainamide column flow-through; lane 7, molecular weight marker (Bio-Rad); lane 8, procainamide column elution.