TABLE 3.
Distribution of penicillin MICs for N. gonorrhoeae isolates with different genotypes
| Genotypea | No. (%) of isolates | No. of isolates with MIC (μg/ml)
|
No. (%) of isolates by susceptibility levelb
|
P valuec | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.015 | 0.03 | 0.06 | 0.12 | 0.25 | 0.5 | 1 | 2 | 4 | 8 | 16 | S | I | R | |||
| wt | 54 (11.6) | 29 | 16 | 4 | 4 | 1 | 49 (90.7) | 5 (9.3) | 0 | <0.001 | ||||||
| penAmut | 51 (9.4) | 19 | 1 | 4 | 13 | 4 | 6 | 2 | 25 (49.0) | 24 (47.1) | 2 (3.9) | >0.05 | ||||
| mtrRmut | 1 (0.2) | 1 | 1 (100) | 0 | 0 | |||||||||||
| penBmut | 2 (0.4) | 2 | 2 (100) | 0 | 0 | |||||||||||
| penAmutpenBmut | 7 (1.5) | 2 | 1 | 4 | 2 (28.6) | 5 (71.4) | 0 | >0.05 | ||||||||
| penAmutmtrRmut | 15 (3.2) | 2 | 4 | 4 | 2 | 3 | 1 | 1 | 5 (33.3) | 9 (60.0) | 1 (6.7) | >0.05 | ||||
| penAmutpenBmutmtrRmut | 6 (1.3) | 1 | 2 | 2 | 1 | 1 | 3 (50.0) | 3 (50.0) | 0 | >0.05 | ||||||
| penAmutponAmut | 39 (8.4) | 2 | 2 | 2 | 13 | 4 | 9 | 5 | 2 | 6 (15.4) | 31 (79.5) | 2 (5.1) | <0.001 | |||
| penAmutponAmutmtrRmut | 62 (13.4) | 4 | 2 | 7 | 7 | 21 | 17 | 4 | 1 | 6 (9.7) | 51 (82.3) | 5 (8.0) | <0.001 | |||
| penAmutpenBmutponAmut | 76 (16.4) | 2 | 3 | 1 | 11 | 9 | 17 | 27 | 6 | 1 | 6 (7.9) | 63 (82.8) | 7 (9.2) | <0.001 | ||
| penAmutpenBmutponAmutmtrRmut | 133 (28.7) | 6 | 5 | 4 | 11 | 9 | 33 | 40 | 18 | 4 | 1 | 15 (11.3) | 95 (71.4) | 23 (17.3) | <0.001 | |
| blaTEM-1pres | 18 (3.9) | 3 | 3 | 2 | 4 | 6 | 0 | 3 (16.7) | 15 (83.3) | <0.001 | ||||||
| Total | 464 | 66 | 29 | 25 | 63 | 36 | 92 | 98 | 36 | 7 | 6 | 6 | 120 (25.9) | 289 (62.2) | 55 (11.9) | |
Pattern of known genetic markers selected for analysis of antimicrobial resistance to penicillin. wt, wild-type (native) sequence in loci associated with resistance to penicillin; mtrRmut, nucleotide substitutions are found in either the mtrR gene or its promoter; blaTEM-1pres, combination of blaTEM-1 gene presence with any variants of other genetic loci.
S, susceptible strains; I, intermediately resistant strains; R, resistant strains (in accordance with CLSI criteria [3] for certain antibiotics). Nonsusceptible categories are shown in bold.
Significance of distinctions between isolates from different phenotypes for the particular genotype examined (two-tailed Fisher's exact test was used).