FIG. 1.

PPMO target sites in viral RNA and sequence conservation in the stem-loop 5 region of human entero- and rhinoviruses. (A) Schematic of a composite entero- and rhinovirus genome. The VPg protein is indicated by a black dot at the 5′ end of the genome; and the names of other features of the viral genome are in italicized font, including the cloverleaf region (′CL') and the other five stem-loop regions of the 5′ UTR (denoted with roman numerals), the initiator AUG, and the poly(A) tail. The bracketed region before the AUG represents the ∼100-nt difference in length between the HRV and PV1 5′ UTRs. PPMO target sites are in nonitalicized font and are indicated by thin lines and arrows. (B) Histogram showing sequence conservation in the stem-loop 5 region of human entero- and rhinoviruses. A total of 196 full-length genomic sequences were aligned and analyzed (see Materials and Methods). An arbitrary scale is depicted at the left of the histogram, where 1.0 denotes perfect agreement at a nucleotide position across all entries. The relative target sites of the two versions of EnteroX and the PV-L5 PPMO are shown above the histogram.