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ToPro3 assay showing very similar permeability profiles for daptomycin and the control. Uptake of ToPro3 was monitored by fluorescence-activated cell sorting following treatment with daptomycin (5 μg/ml), ciprofloxacin (2 μg/ml), nisin (25 μg/ml), CCCP (10 μM), and A23187 (1 μg/ml).
