FIG. 3.

β-Peptides inhibit HSV-1 infection in a comprehensive assay. (A) Vero cells. (B) HK320 cells. Confluent cells in a 96-well plate and recombinant hrR3 KOS virus were incubated with various concentrations of the β-peptides for 1 h at 37°C prior to infection. Treated cells were then infected with treated viruses and incubated at 37°C for 6 h. Infected cells were lysed, chlorophenol red-β-galactopyranoside was added, and the kinetics of β-galactosidase (β-gal) activity, as a measure of infectivity, was then determined. The activity observed in cells that had not been exposed to a β-peptide was set to 100% infectivity. Each data point and error bar represent the mean ± standard deviation of results for triplicate samples. ○, peptide 1; •, peptide 2; ▾, peptide 3.