FIG. 3.
Comparison of the susceptibility level of the parental cell line with that of the cell line resistant to ACH-806. The dose responses of ACH-806 in Huh-9-13 cells, a parental cell line, and in ACH-806R, a clone (no. 28) derived from Huh-9-13 under the selection of ACH-806. Both cell lines were treated in duplicate with ACH-806 at the concentrations indicated for 3 days. The HCV replicon level following the treatment was quantified with a dot blot assay using a 32P-labeled riboprobe as described in Materials and Methods. (A) Autoradiograph of the membrane. (B) Dose-response curve. The fitting was performed based on the radioactivity (cpm) in each spot on the membrane relative to that of the untreated controls (100%) by using nonlinear regression with Prism software (GraphPad Software, Inc., San Diego, CA).