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. 1999 Nov 23;96(24):13926–13931. doi: 10.1073/pnas.96.24.13926

Figure 1.

Figure 1

(A) Expression of TdT in Sf9 cells. Sf9 cells were infected with recombinant baculovirus expressing TdT (lane 2); TdT and His-Ku70 (lane 3); TdT and His-Ku86 (lane 4); TdT and His-Ku70/86 (lane 5); and TdT and adenosine deaminase (ADA; lane 6). The baculovirus constructs used for infections are indicated above the lanes. Lane 1 contains 30 ng of purified TdT. Extracts were made 72 h after infection, electrophoresed on SDS/10% PAGE, and transferred to a PVDF membrane. The blot was probed with rabbit anti-TdT polyclonal antibody at a 1:1,000 dilution and developed by using the enhanced chemiluminescence method. (B) Coimmunoprecipitation of Ku subunits with TdT. Approximately 50 μg of extract obtained from single, double, or triple coinfections was incubated with the anti-TdT 133D2 (lane 2), anti-polyHis (lanes 3–5), and anti-ADA N1D1 (lane 6) mouse mAbs. The immunoprecipitated complexes were resolved by SDS/10% PAGE, transferred to PVDF, and probed with rabbit anti-TdT polyclonal antibody (1:1,000 dilution). The blots were developed as described in A.