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. 1999 Nov 23;96(24):13926–13931. doi: 10.1073/pnas.96.24.13926

Figure 2.

Figure 2

(A) Expression of Ku subunits in Sf9 cells. Sf9 cells were infected with recombinant baculovirus expressing TdT and His-Ku70 (lane 1); TdT and His-Ku86 (lane 2); TdT and His-Ku70/86 (lane 3); and Ku70/86 (lane 4). Extracts obtained 72 h after infection were electrophoresed on SDS/10% PAGE and transferred to a PVDF membrane. The blot was probed with anti-polyHis antibody (1:5,000 dilution) and developed by using the enhanced chemiluminescence method. (B) Coimmunoprecipitation of TdT with Ku subunits. Approximately 50 μg of extract obtained from single, double, or triple coinfection experiments was incubated with either the anti-polyHis (lanes 1–3) or anti-TdT 133D2 (lanes 4–6) mAbs. The immunoprecipitates were resolved by SDS/10% PAGE, transferred to PVDF membranes, and probed with anti-polyHis (1:5,000 dilution) antibody. The blots were developed as described in Fig. 1. IP, immunoprecipitation.