T cells from T-GFP mice express GFP uniformly and selectively. To
generate T-GFP mice, the GFP coding sequence was placed under control
of the murine CD4 promoter/proximal enhancer without the intronic
silencer and the plasmid construct (A) was microinjected
into fertilized oocytes. (B) Splenocytes from T-GFP mice
were stained with mAbs against murine T cells (Thy1.2), CD4, CD8, B
cells (B220), macrophages (Mac-1), and NK cells (NK1.1) and examined by
flow cytometry. Examination of peripheral blood leukocytes revealed
that Gr-1+ granulocytes were also GFP− (not
shown).