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. 1999 Nov 23;96(24):13932–13937. doi: 10.1073/pnas.96.24.13932

Figure 1.

Figure 1

T cells from T-GFP mice express GFP uniformly and selectively. To generate T-GFP mice, the GFP coding sequence was placed under control of the murine CD4 promoter/proximal enhancer without the intronic silencer and the plasmid construct (A) was microinjected into fertilized oocytes. (B) Splenocytes from T-GFP mice were stained with mAbs against murine T cells (Thy1.2), CD4, CD8, B cells (B220), macrophages (Mac-1), and NK cells (NK1.1) and examined by flow cytometry. Examination of peripheral blood leukocytes revealed that Gr-1+ granulocytes were also GFP (not shown).