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. 1999 Nov 23;96(24):13938–13943. doi: 10.1073/pnas.96.24.13938

Figure 2.

Figure 2

Anti-CD1d crosslinking induces epithelial release of IL-10; role for the CD1d cytoplasmic tail and tyrosine phosphorylation. T84 cell lines CD1d (wild-type) or CD1a/d chimera (chimeric) cell lines were grown to confluence on permeable supports. Apical CD1d was crosslinked, and soluble cell supernatants were harvested over the indicated periods of time and assayed for IL-8 (A), TNF (B), or IL-10 (C) by ELISA. PMA, phorbol 12-myristate 13-acetate. Results represent the mean ± SEM for 7–10 monolayers in each condition. In D, apical CD1d was crosslinked in the presence of indicated concentrations of genistein, and soluble cell supernatants were harvested 72 h after crosslinking and assayed for IL-10 by ELISA. Results represent the mean ± SEM for 6–8 monolayers in each condition.