Anti-CD1d crosslinking induces epithelial release of IL-10; role for
the CD1d cytoplasmic tail and tyrosine phosphorylation. T84 cell lines
CD1d (wild-type) or CD1a/d chimera (chimeric) cell lines were
grown to confluence on permeable supports. Apical CD1d was crosslinked,
and soluble cell supernatants were harvested over the indicated periods
of time and assayed for IL-8 (A), TNF
(B), or IL-10 (C) by ELISA. PMA, phorbol
12-myristate 13-acetate. Results represent the mean ± SEM for
7–10 monolayers in each condition. In D, apical CD1d
was crosslinked in the presence of indicated concentrations of
genistein, and soluble cell supernatants were harvested 72 h after
crosslinking and assayed for IL-10 by ELISA. Results represent the
mean ± SEM for 6–8 monolayers in each condition.