Figure 3.

Quantification of IL-10 message by PCR amplification. Reverse transcription–PCR was examined in CD1d wild-type (Upper) and CD1a/d chimeric (Lower) cell lines exposed to anti-CD1d crosslinking for indicated periods of time. After crosslinking, total RNA was isolated, treated with DNase I, and amplified by reverse transcription–PCR for IL-10 message or for control β-actin. Data shown represent three experiments.