Figure 4.

Epithelial-cell-derived IL-10 blocks IFN-γ-induced epithelial permeability. T84 cells overexpressing CD1d were grown as monolayers on permeable supports. Some monolayers were anti-CD1d crosslinked (indicated as XL) for 48 h, followed by the addition of IFN-γ (100 units/ml) in the presence and absence of anti-IL-10, as indicated. Monolayer TER was monitored by standard voltage clamp techniques. Results are expressed as the mean ± SD for four individual monolayers in each condition. One of three representative experiments is shown.