Figure 5.

At the cell surface, MHC protein accumulates in distinct microdomains. 221/Cw6-GFP cells were stained with (A) phycoerythrin-labeled TÜ39 mAb (red), (B) TÜ39 mAb followed by Alexa 568 goat anti-mouse IgG mAb (red), (C) TR-transferrin (red), or (D) biotinylated cholera toxin with phycoerythrin-labeled streptavidin (red). No staining was seen with phycoerythrin-labeled streptavidin alone. The green fluorescence of HLA-Cw6-FP, each red fluorescence, and an overlay of both is shown. (Right) Yellow indicates colocalization of green and red staining. Magnification: ×6,000.