TABLE 1.
On-rates, off-rates, and dissociation constants of EGF and wild-type and mutant IFNα2
| Kon | Koff | Kd | Chimeric activators (CA) | |
|---|---|---|---|---|
| 1/m·seg | 1/s | m | ||
| EGF | 1 × 106 | 2.6 × 10–3 | 2.6 × 10–9 | |
| IFNα-2a (wild-type) | 3.7 × 106 | 1 × 10–2 | 3 × 10–9 | IFNα-2a(wild-type)-(Gly4Ser)7-EGF (CA-WT) |
| IFNα-2a (K133A) | 0.7 × 106 | 1.8 × 10–2 | 26 × 10–9 | IFNα-2a(K133A)-(Gly4Ser)7-EGF (CA-K133A) |
| IFNα-2a (R144A) | 0.36 × 106 | 4 × 10–2 | 120 × 10–9 | IFNα-2a(R144A)-(Gly4Ser)7-EGF (CA-R144A) |
| IFNα-2a (R149A) | Too low to measure | 538 × 10–9 | IFNα-2a(R149A)-(Gly4Ser)7-EGF (CA-R149A) |