TABLE 2.
Low molecular weight peptides identified in the young, aged, and cataract human lenses using MALDI-TOF MS and quadrupole time-of-flight MS/MS
Peptides shown in boldface type were used in the current study. The peptide sequence was numbered based on the GenBank™ accession number given in the table.
|
Peptide mass
|
Source
|
Accession number
|
Found in
|
Peptide sequence from MS/MS analysis
|
||
|---|---|---|---|---|---|---|
| Young | Aged | Cataract | ||||
| 922.43 | αB-(59–66) | NP_001876 | No | Yes | Yes | PSWFDTGL |
| 1152.64 | αA-(67–75) | NP_000385 | Yes | No | No | DRDKFVIFL |
| 1239.67 | αA-(66–75) | NP_000385 | No | Yes | Yes | SDRDKFVIFL |
| 1381.64 | αB-(24–34) | NP_001876 | No | No | Yes | FDQFFGEHLLE |
| 1388.77 | γS-(167–178) | NP_060011 | Yes | Yes | Yes | SPAVQSFRRIVE |
| 1426.75 | αA-(2–12) | NP_000385 | No | Yes | Yes | DVTIQHPWFKR |
| 1527.81 | αA-(2–13) | NP_000385 | No | Yes | Yes | DVTIQHPWFKRT |
| 1550.84 | αB-(45–57) | NP_001876 | Yes | Yes | No | SPFYLRPPSFLRA |
| 1730.96 | αA-(43–56) | NP_000385 | Yes | Yes | Yes | TISPYYRQSLFRTV |
| 1778.91 | αA-(67–80) | NP_000385 | No | Yes | Yes | DRDKFVIFLDVKHF |
| 1835.01 | αA-(51–65) | NP_000385 | No | No | Yes | SrLFRTVLDSGISEVRa |
| 1847.99 | αA-(66–80) | NP_000385 | No | Yes | Yes | SDRDKFVIFLDVKHF-H20 |
| 1865.94 | αA-(66–80) | NP_000385 | No | Yes | Yes | SDRDKFVIFLDVKHF |
| 1938.43 | βA3/A1-(59–74) | AAC50971 | No | Yes | Yes | SD(N)AYHIERLMSFRPIC |
| 1963.07 | αA-(50–65) | NP_000385 | No | No | Yes | QSrLFRTVLDSGISEVRa |
| 2064.09 | βB2-(181–196) | AAB25691 | Yes | No | Yes | PQVQSVRRIRDMQWHQ |
| 2187.16 | αB-(2–18) | NP_001876 | No | Yes | Yes | DIAIHHPWIRRPFFPFH |
| 2359.18 | αB-(1–18) | NP_001876 | No | Yes | Yes | Ace-MDIAIHHPWIRRPFFPFH |
| 2598.35 | β(γ)S-(2–22) | P22914 | No | Yes | Yes | Ace-SKTGTKITFYEDKNFQGRRYD |
| 2930.62 | βB2-(181–204) | AAB25691 | Yes | No | Yes | PQVQSVRRIRDMQWHQRGAFHPSN |
| 3196.99 | βA3-(190–215) | NP_005199 | Yes | Yes | Yes | GDYKHWREWGSHAQTSQIQSIRRIQQ |
| 3252.65 | βA3-(189–215) | NP_005199 | Yes | Yes | Yes | GGDYKHWREWGSHAQTSQIQSIRRIQQ |
| 3389.74 | βA3-(188–215) | NP_005199 | Yes | Yes | Yes | HGGDYKHWREWGSHAQTSQIQSIRRIQQ |
a
MS/MS reported an extra Arg residue, which is shown as a lowercase letter