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. 2008 Mar 28;283(13):8395–8405. doi: 10.1074/jbc.M709456200

FIGURE 5.

FIGURE 5.

Inhibition of SALM4 clustering by an antibody to LRR. A, Myc-SALM4 was transfected into HeLa cells, and surface expression was detected using an antibody directed against the SALM leucine-rich repeats (anti-LRR). Intracellular SALM4 was detected with anti-Myc (see Total). Surface staining indicates that the LRR antibody recognizes SALM4 intercellular clusters (arrowhead). B, HeLa cells transiently transfected with Myc-SALM4 were treated for 24 h with antisera directed against the SALM LRR or SALM4 C terminus (anti-S4CT), which served as a control. SALM4 surface staining was detected by anti-Myc, and intracellular SALM4 was detected by anti-S4CT antibodies. SALM4 cluster formation is largely inhibited by anti-LRR (1:250). However, SALM4 surface clustering is not disrupted by application of anti-S4CT (1:250). C, treatment of Myc-SALM4-transfected HeLa cells with anti-Myc antibodies (1:1000) for 24 h enhances SALM4 clustering (arrowheads). Anti-Myc antibodies also induce clustering of the SALM2 C terminus (arrowheads, bottom panel) in HeLa cells transfected with full-length Myc-SALM2 (detected with anti-S2CT).