FIGURE 8.

Integration of BMP2 osteogenic signal by RUNX2 during differentiation requires RUNX2-SMAD interaction. Runx2^-/- cells were cultured in 6-well plates and 3 days later infected with the pre-determined m.o.i. of indicated Runx2 adenoviruses. Cells were fed every other day with osteogenic media in the presence or absence of 100 ng/ml BMP2. Cells were harvested at the indicated days, and RNA was isolated. Real time-PCR for selective marker genes were carried out in duplicate, and values were normalized to internal glyceraldehyde-3-phosphate dehydrogenase levels in the same sample. Data are presented as -fold induction (virus-infected BMP2-treated/virus-infected control).