TABLE 3.

Phosphorylation of OmpR and OmpR mutant proteins

The amino acid substitution is listed in the 1st column. The percent of the protein that was phosphorylated by incubation with acetyl phosphate for 60 min is compared in the 2nd column. In the 3rd column, this value is converted to a percent where the phosphorylation of the wild type protein represents 100%. Wild type and mutant proteins were phosphorylated and analyzed by HPLC as described under “Experimental Procedures.”

	Phosphorylation by acetyl phosphate after 60 min	Phosphorylation compared with wild type
	%	%
Wild type	77.3	100
T224C	7.5	10
V225C	31.2	40
W226C	13.9	18
Wild type + dithiothreitol	79.8	100
W226C + dithiothreitol	72.0	90